3D Transfection: a novel perspective for your cells !

3D-FectIN™ is the newest reagent specifically developed to directly transfect cells cultured in 3D hydrogel. 3D-FectIN™ is suitable for all kind of hydrogels and cells. This reagent is based on a novel technology that allows adding a third dimension to cell culture.

3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behavior. Because 3D matrices are routinely used in basic research and therapeutic applications, we have developed 3D-FectIN™ transfection reagent specific for hydrogel. In this way, hydrogel-based 3D matrices combined with 3D-FectIN™/DNA complexes, are colonized by cells to be transfected in a more natural environment. This method allows studying angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation…
 

• Highly efficient: on cell lines and primary cells
• Specific for 3D hydrogels
• All type of nucleic acids: plasmid DNA, siRNA, shRNA...
• Completely biodegradable
• Serum compatible
• Long term protein expression

High transfection efficiency in various cells

3D-FectIN™ has been successfully tested on a variety of cells. Transfection efficiency and transgene expression achieved are high and long-lasting.

Transfection of various cells on different gels

High secreted alkaline phosphatase expression

Efficient with various type of hydrogels and matrices

Outperforms competitors

Comparison of RFP expression in HEK-293 cells

Comparison of secreted alkaline phosphatase production in NIH-3T3 cells