Monoclonal Antibodies (murine)

ABC scientific, in collaboration with Biotem, a worldwide recognized company in the field of custom antibody production, offers custom murine monoclonal antibodies.

 

Hybridoma Development

Project specification exploration

Target Typology	Initial materials and project constraints
Virus, bacteria, etc. Proteins (intracellular, membrane, post-translational modifications, etc.) Peptides (design, synthesis and conjugation) Chemical compounds and hapten	Material Available (amount , purity) Immunogenicity / Toxicity Cross reaction and specificity Deadlines

 

 Applications

• ELISA, WB, IP, IF, IHC, FACS, etc.
• Functional testing, quality controls.
• Lateral Flow, etc.

Biotem has developed a large panel of improved immunisation/fusion technologies enabling us to complete the most challenging projects. As a first commitment, we ensure x positive clones and 30 % of viability (16h after thawing) in output of fusion.

Customised Immunisation & Fusion Strategies

Protocol R.A.D.®: Rapid Antibody Development     (≈1 month) Standard Protocols Genetic (DNA based), Subtractive, In vitro, etc. Specific protocols available for: Small size & poorly immunogenic compounds Tiny quantities of immunogen (<300 µg) High affinity antibodies

 

The development of monoclonal antibodies includes several key screening stages. BIOTEM carries out the most relevant screening/cloning strategy.

After the cloning step, BIOTEM assures a second commitment: 90 % of monoclonality and 50 % of viability (16h after thawing).

Hybridoma Selection

• Validated and optimised primary screening: false positive/negative reduced.
• Robust hybridoma screening using proprietary Cyclop®Screening System
• Additional tests for confirmation: ELISA, WB, FACS, etc. by BIOTEM and customers

Hybridoma Cloning & Banking

• Several cloning cycles for extensive hybridoma stabilisation
• Multiple sub-clones generation
• Validated cell bank with monitored viability

Antibody Production

We provide high quality antibody production thanks to both in vivo and in vitro protocols. The in vitro culture techniques include several processes for a production up to 20g. Additionally, Ascites production from nude mice often offers higher yields concentrations of antibody and allows eliminating the endogenous mouse IgG

 

We provide high quality antibody production thanks to both in vivo and in vitro protocols.
The in vitro culture techniques include several processes for a production up to 20g.
Additionally, Ascites production from nude mice often offers higher yields concentrations of antibody and allows eliminating the endogenous mouse IgG.

Antibody Purification & Modifications

• Chromatography: Affinity, Ion exchange, size exclusion, hydrophobic
• Specific methods for IgM
• Conjugation, fragmentation
• Endotoxin free batches upon request
• Etc.

Additional Services

The know-how and the experience of our scientists enable us to develop highly customised projects.
Epitope mapping , antibody sequencing (CDRs & full antibody)
Affinity and kinetic constants determination (Kd, Kon, Koff)
Stability study, quality controls, etc.