An innovative transfection technology

Several optimized reagents have been designed according to define applications:

• PolyMag Neo : A new reagent for all nucleic acids transfection
• PolyMag: for all nucleic acids transfection
• CombiMag: for enhancing all transfection reagents efficiency
• ViroMag and ViroMag R/L : for virus transduction
• AdenoMag : for adenovirus & AAV transduction NEW
• SilenceMag : for siRNA applications
• Neuromag : for Neurons transfection

Magnetic nanoparticles for nucleic acids and small molecules delivery

This method associates nucleic acids or other vectors with magnetic nanoparticles coated with cationic molecules. The magnetic nanoparticles are made of iron oxyde, which is fully biodegradable, coated with specific cationic proprietary molecules varying upon applications. Their association with the gene vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced colloidal aggregation and electrostatic interaction.

Intracellular uptake of nanoparticles

The resulting molecular complexes are then targeted to and endocyted by cells, supported by an appropriate magnetic field. Membrane architecture and structure stay intact in contrast to other physical transfection methods that damage, create hole or electroshock the cell membranes. In addition the magnetic nanobeads are completely biodegradable and not toxic at the recommended doses and even higher. In this manner, the complete applied vector dose gets concentrated on the cells within a few minutes. Consequently, 100% of the cells get in contact with a significant vector dose.

Intracellular release of vectors and drugs

The nucleic acids or other vectors are then released into the cytoplasm by different mechanisms depending on the formulation used. One is the proton sponge effect caused by cationic polymers coated on the nanoparticles that promotes endosome osmotic swelling, disruption of the endosomal membrane and intracellular release of associated molecules. Second is the destabilization of the endosome by cationic lipids coated on the particles that release the vectors into cells by flip-flop of cell negative lipids and charged neutralization. Third one is the usual viral mechanism when virus is used.

Inspired by the validated and recognized magnetic drug targeting technology, this innovative method is a revolution for transfection and infection.

MagnetoFection™ applications and results

MagnetoFection™ has been successfully tested on a broad range of cells and cell lines. It is perfect for hard-to-transfect cells, primary cells and non dividing or slowly dividing cells. We have shown that combining magnetic nanoparticles to gene vectors of any kind results in a dramatic increase of uptake of these vectors and high transfection or delivery efficiency.

Primary Human Keratinocytes transfection	Primary Pig Chondrocytes transfection

siRNA delivery in HUVEC with SilenceMag	Adenovirus infection with ViroMag

MagnetoFection™ advantages

Non toxic method

Rapid and efficient even with low DNA doses

MagnetoFection™ equipment

Super Magnetic Plate	Magnetic plate with 96 magnets	Mega Magnetic plate
Convenient for all cell culture support Catalog number #MF10000	Adapted to 96-well plates Catalog number #MF10096	To hold 4 culture dishes at one time #MF14000

Rapid and straightforward protocol